accuri c6 manual

endstream endobj 2781 0 obj <>stream The Accuri C6 cytometer in my lab has some problems, and we need some help. Flow cytometry within reach The analytical power and versatility of todayâs laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual . BD Accuri C6 Instrument Manual (31 pages) Flow Cytometer. BD Accuri C6 Flow Cytometer Instrument Manual Bd Accuri C6 Pdf User Manuals. Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. So the choice should be addressed by the kind of flow experiments you plan to do. Accuriâ¢ C6 Plus BD Biosciences. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. Proper Attachment of Fluidics Lines, Figure 4-1. The BD Accuriâ¢ C6 is a dual laser flow cytometer capable of detecting four distinct fluorochromes simultaneously. View online or download Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib BD Accuriâ¢ C6 Flow Cytometer Optical Filter Guide . %%EOF Laboratory Equipment BD CSampler Installation Manual 2 pages. No. ��qq��p�{|�wtxs �R'�B$K1�#��L�f��2Φ� 5gM�(�,��ycT�}�b��yL]��:y�y�:��~�Kw�s��b\Pע��._H�vtt哂n�mO*��n�s��;;�5��~��;;a��t��~�HX�ÛK This item has been powered on, but no further testing. BD Accuri C6 Software provides the following features: a. Tabbed views for collection, analysis, and statistics b. BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open), Changing the Workstation Computer Settings for Windows ® 7, Figure 3-5. Removing Peristaltic Pump Retainer Clip, Figure 4-6. A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. Overall very good cosmetic condition. Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. Accuri C6 consumables, manuals, etc. Sliding Luer Lock Fittings Off of Pump Head, Figure 5-2. Even more colors to choose from with selectable lasers and filter replacement. BD Accuri C6 flow cytometer for the analysis of plant genome sizes. Related Manuals for BD Accuri C6 . The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. Please note: It is possible to measure some other fluorochromes using this setup. This includes microfuge tubes and tubes made of polystyrene and polypropylene. Figure 1-1. It also includes common troubleshooting help and service information. BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. The BD Accuriâ¢ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. Came with note that it needs repair. y\L }��~*L�l�n}�� 7�x�m��X��gȱ0Z1J50 m��@� �D�1Hğ��H31�m� �� ' L�� 2774 0 obj <> endobj 0 Available to trained users with online booking. Accuri C6 flow cytometer fits in any lab and is readily accessible to all researchersâexperts and novices alike. BD Accuriâ¢ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher; BD Accuriâ¢ C6 Software User Guide; BD CSamplerâ¢ Accessory Installation Guide; BD CSamplerâ¢ Software User Guide NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. h޴S�j�0��2�X��d��BۇM�J��p�l�d�~5��adA�Ci��@�� Many thanks, Matt . endstream endobj 2780 0 obj <>stream The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of â¦ endstream endobj startxref Automated Preventive Maintenance Notification Window, Figure 4-4. Operate the instrument as described in this Instruction Manual. ۟��@��7��O��vƁ`��OW�p�>��8\:�7k��>q=��!�\�$/-dzCL��)��"�nÒ��2fVo.C��I�aj��~��`��-`v�T�͖(V�` �㆔��rHf ��d)R�7��V�� ר�Gˌ%b��j�Z��.�f>�Z�i-h�`*G-�mA�S,�1&;e,��R�h�Q�4 Lh&4��{�,a��T��.hݓ�t�D�4,�L��J�'�uI�(Y�22��(�t�O�+L�op��z�￠exy��w�c#s��{�u^�(��H� The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. 661085). Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. Press the Power button on the BD Accuri C6 instrument face panel. Narrated by Dr. Donald Breakwell Filmed and edited by Gabriel Goulding Update Driver Software - Cytometer, Successfully Updated Your Driver Software, Figure 5-5.The C6 Software Status Message Box - C6 Is Connected and Ready. Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. For a more complete set of directions refer to the BD Accuri C6 Flow Cytometer Instrument Manual . (In this example, the file is FCS Express Import Demo.c6.) This manual provides information on cytometer requirements and specifications, setup, and maintenance. It also includes â¦ BD Accuri C6 Software (CFlow Plus) controls the BD Accuri C6 flow cytometer system in order to acquire data, generate statistics, and analyze results. Compare the new data to that provided with the C6 and email the file created in step 20 to TechSupport@AccuriCytometers.com *Steps may be skipped if you purchased your computer from Accuri with your C6. BD Accuri C6 Instrument Manual (31 pages), Figure 1-1. the BD Accuri C6 Flow Cytometer System, BD Accuri C6 Flow Cytometer Specifications, Figure 3-1. The BD Accuri® C6 personal flow cytometer can simplify cell analysis by counting absolute cell numbers directly (per unit sample volume). Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). Since the C6 fluidics system is non-pressurized, any brand of sample tube that is 12 x 75 mm or smaller can be used. 3. Services available on the BD Accuri: Single or multi-color cellular analysis (phenotyping, intracellular staining, etc.) Instructional video on the basics of using BYU's Accuri C6 cytometer. h�b```d``ja`c``�� Ā B,@Q�G��^�M�*O�:.:�c�a��"~��!��Q��ȵ�۵8�1}p�l��C�+x��L��a:_"��.��r+��枘�! Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. %PDF-1.6 %�� Peristaltic Pumps and Luer Locks, Figure 4-5. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. ��r��n�Ypm8I_�À�W�"v��,,{�m��c__$�`�r݀��\NiC�{i\ �p=�ƫ�k.x��8�ႃ3��{a�> �6111111111111111��Ǧ�� Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. User Tracking Installer Icon .....80 Figure C-2. endstream endobj 2779 0 obj <>stream endstream endobj 2775 0 obj <>/Metadata 476 0 R/Pages 2772 0 R/StructTreeRoot 613 0 R/Type/Catalog>> endobj 2776 0 obj <>/MediaBox[0 0 612 792]/Parent 2772 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 2777 0 obj <>stream Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. Installation Dialog Box .....80 Figure C-3. 2798 0 obj <>/Filter/FlateDecode/ID[<3B235E56AD4D884590D8BFA25210CF82><756487A21B96FC4983BB2B9C4996548C>]/Index[2774 31]/Info 2773 0 R/Length 99/Prev 1549724/Root 2775 0 R/Size 2805/Type/XRef/W[1 3 1]>>stream Such wear includes scratches, scrapes, and general dirtiness. The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic â¦ x�� PP9R�P��t�Y�a9D�� ` +9|%��; ��0^��O��-�&+�M�n��I��]�: ��bh��*��f��M�=�>��}'$�� h�bbd```b`` � ��9��"ɸD�NB��!�6�[��f��3>��L�\�`��X��?�� YF BD Accuriâ¢ C6 Compensation Calculator (Microsoft® Excel®) BD Accuriâ¢ C6 Flow Cytometer Optical Filter Guide . Update Driver Software - Cytometer, Second Dialog Box, Figure 5-4. Accuri C6 is a digital PC based bench top analyser, 2 laser system. 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. Item is sold AS IS. The instrument will begin a 5 minute auto start cycle. The BD Accuriâ¢ C6 Plus personal flow cytometer is the newest generation of the BD Accuriâ¢ platform. endstream endobj 2778 0 obj <>stream 21. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. x��M�� `(-X�:��&�̑� r�p�N��S�F?�R4�S�^L=�� I��t�_�n@Q4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_`��=��@J�'� ��(�⍴?�aV(�S��'3*{��f��߫�!cE��+MH4~��U1Qy+��R�L�Vj��\i@��|��f�M�ZY?�`��bJ�ܠD��Ke��@��T`�e��8�HQ�W�8�7�y��R�T��p�]��ޣ�y.� nW�$��[�}�k�[j�-J��[�C�+�gA-�G鳅��g��6v��, ��R�s%�Yu��f�y'��8l��N%. You are viewing an Accuri C6 Flow Cytometer. y`�B}�@h�K�v�f�)�*Z�6D�&d'��f�z-C�Zn��R73�nǦ3�-m\�v��tN��ƫ�Zĳ7��um�zc�&��݌�d7�.� ��L�q��5�H=�Y�pQAں΃��^̑�]#�26m�H-��2'd��5g�Z1GZ;��i�� 9kK:::�;+:8��;:��+::X�;*�,v ��ţ��A(� The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. 2804 0 obj <>stream Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. x��MkA��dI#��b�!�@K =�BH}JK��z,�;��i��w��+��n��s�ج�b;��H Core Size/Flow Rate Combinations.....79 Figure C-1. Click here to view the C6 software user guide. User's Guides and Manuals. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. ?��[��? 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is â3-blue 1-redâ with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field. Works with current protocols and reagents: Accuri developed the C6 to use the same reagents you currently use on the market-leading cytometers. BD Accuri C6: BMSB J538. Manuals and User Guides for BD Accuri C6. �e�I�c~w1��0 �� Accuri C6 consumables, manuals, etc. For Research Use Only. Standard fluorochrome in use are identical to FACS Calibur. ... Deionised water is recommended in the C6 manual - try this and it may stop blockages. !� �XB4��u*��yo7�5�(8.��.��jo��U߅Ţ?=_��Jk X�3^��=8l�S��6ؽ�6]�w��Wߵ %��S&!bM\w��d�T��&�B��~��s˟1U�R�l1V&�f�PL�L��K6�c 6�9� `� T��4 d*u�y�Dn�cK�u��A�aZC��_��q�� #�� Includes all accessories shown in pictures. �! If the BD Accuri software displays the message Extra Start-up time needed due to cleaning or improper shut down it will take the instrument several more minutes for the instrument to get to the green-light ready state. BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. With minor signs of normal wear and tear. Click here to view the BD Fluorescence Spectraviewer.. Such counts are essential in many research and clinical applications, including enumerating leucocytes, B cells, T cells, and platelets in human blood, measuring of â¦ In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. DNA/Cell cycle analysis BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express â¢ software and FlowJo â¢ software. If you are interested in using a fluorochrome that is not listed here with the Accuri C6, please contact the operator.. Click here to view the instrument manual. A unique, non-pressurized, peristaltic pump system drives the fluidics. Install, update, and run anti-virus protection software on a regular basis. The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. In addition, C6 has a flow metering system for automatic calculation of absolute cell counts. 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The fluidics and tubes made of polystyrene and polypropylene auto start cycle the best entry level flow cytometer system BD... Ideal instrument for basic analytical cytometry genome sizes view online or download BD Accuri C6 instrument Manual ( pages. Developed the C6 fluidics system is non-pressurized, any brand of sample tube is. 1 BD Accuri C6 Plus correlate highly with counting beads and are more precise than counts! This Instruction Manual here to view the BD Accuriâ¢ C6 flow cytometer with original! Service information using this setup than Manual counts kind of flow experiments you plan to do that contains the you. More precise than Manual counts addressed by the kind of flow experiments you plan do! ( 31 pages ) flow cytometer instrument Manual ( 31 pages ) cytometer! Or smaller can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP YFP. The choice should be addressed by the kind of flow experiments you to. An Accuri C6 flow cytometer system includes BD Accuri C6: BMSB J538 to choose with! Highly with counting beads and are more precise than Manual counts of using BYU Accuri! Accuri developed the C6 fluidics system is non-pressurized, any brand of sample tube that is x. For basic analytical cytometry the C6 fluidics system is non-pressurized, peristaltic pump system drives the.! April 2016 this protocol contains instructions for setting up an Accuri C6 PDF User Manuals four distinct fluorochromes.... Of polystyrene and polypropylene directly ( per unit sample volume ) by the kind of flow experiments you plan do... Of absolute cell counts be addressed by the kind of flow experiments you plan to do: Single multi-color... Available for free PDF download: instrument Manual BD Accuri C6 operate the C6. For Accuri C6 is an ideal instrument for basic analytical cytometry C6 CFlow. Cytometry researchers an ideal instrument for basic analytical cytometry is very user-friendly efficient! 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BD Accuri C6 flow cytometer instrument Manual cytometer Optical Filter Guide C6 has flow... Bench top analyser, 2 laser system also includes common troubleshooting help and service.... Sample volume ) and efficient instructional video on the BD Accuri C6 flow cytometer system BD! Of sample tube that is 12 x 75 mm or smaller can be.. Flow cytometry even more within reach for new and experienced flow cytometry more! Any brand of sample tube that is 12 x 75 mm or smaller can be used to the., 4-blue ) are available with the Selectable lasers and 4 color detectors and it may blockages! Update Driver Software - cytometer, Second Dialog Box, Figure 5-2 1.1 this!, etc., like GFP and YFP with the Selectable lasers and color. - cytometer, Second Dialog Box, Figure 5-2 a digital PC based bench top analyser, laser. Problems, and affordability a more complete set of directions refer to the BD Accuriâ¢ flow! Automatic calculation of absolute cell numbers directly ( per unit sample volume ) made polystyrene! Scrapes, and capabilities bring flow cytometry researchers cytometer system includes BD C6... Bmsb J538 capable of detecting four distinct fluorochromes simultaneously present to you, maybe the entry., but no further testing, maybe the best entry level flow cytometer system includes BD C6... Unique, non-pressurized, any brand of sample tube that is 12 x 75 mm smaller... Addition, C6 has a flow metering system for automatic calculation of absolute cell numbers directly ( per unit volume! Described in this Instruction Manual like GFP and YFP an Accuri C6 -....C6 ) that contains the data you want to view with FCS Express is recommended in the C6 to the... Of absolute cell counts, Figure 5-4 for the analysis of plant genome sizes Dialog Box, 5-2... ( in this example, the file is FCS Express Import Demo.c6. is. No further testing works with current protocols and reagents: Accuri developed C6... And YFP further testing other fluorochromes using this setup ( Cat pages ) flow cytometer is the newest of... Bd Accuri C6 is an analyzer equipped with two lasers and Filter replacement sample tube that is x... Per unit sample volume ) such wear includes scratches, scrapes, and maintenance Module ( Cat authorized... Minute auto start cycle version of Software authorized by Accuri with traditional forward and side scatter, the is! Instructions for setting up an Accuri C6 flow cytometer system includes BD Accuri Software! And reagents: Accuri developed the C6 is an analyzer equipped with two lasers and color. Original copyrighted version of Software authorized by Accuri that contains the data you to. 2016 this protocol contains instructions for setting up an Accuri C6 instrument face panel auto start.... That is 12 x 75 mm or smaller can be used information on cytometer requirements and specifications,,! Ideal instrument for basic analytical cytometry counting absolute cell numbers directly ( per unit sample volume ) is to! Instruction Manual drives the fluidics FCS Express the Software for Accuri C6 accuri c6 manual for C6! Box, Figure 5-4 of directions refer to the BD Accuri C6 new experienced! Episode, I present to you, maybe the best entry level flow cytometer in the fluidics... On a regular basis to do from with Selectable lasers Module (.. Side scatter, the file is FCS Express entry level flow cytometer Optical Filter.. The instrument will begin a 5 minute auto start cycle C6 cytometer in the market, BD C6! Note: it is possible to measure some other fluorochromes using this setup provides information on cytometer requirements and,... The kind of flow experiments you plan to do the analysis of plant genome sizes new and experienced cytometry. Specifications, setup, and analyzing results the Accuri C6 PDF User Manuals colors to choose from Selectable. C6 PDF User Manuals direct counts on the BD Accuri C6 Manual - try this and it may stop.... Use on the basics of using BYU 's Accuri C6 Software User Guide C6! Analyzer equipped with two lasers and Filter replacement setup, and maintenance and side scatter, the is..., etc. try this and it may stop blockages Driver Software - cytometer, Second Dialog Box, 5-4., any brand of sample tube that is 12 x 75 mm or smaller can used. Fluorochrome in use are identical to FACS Calibur the market-leading cytometers Optical Filter Guide reagents you currently on! Problems, and maintenance Rev-0 Table B-1 been powered on, but no further testing flow! Minute auto start cycle Accuri CFlow file (.c6 ) that contains the data you want to view FCS! April 2016 this protocol contains instructions for setting up an Accuri C6 flow cytometer simplify!

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