→ May require cross-laser compensation depending on fluorophores used → Can replace selected energy transfer (tandem) conjugates. Comparative cytograms of Fluo-4 fluorescence of C6 glioma cells over time, showing the effects of adding control and test compounds (ionophore A23187, ethanol, and thapsigargin). Coastal and bay water contained a variety of algae with distinct autofluorescence signatures, while chlorinated pond water contained a single dominant population. 14.7 mW 640 nm Diode Red Laser BD Accuri™ personal flow cytometers bring immunology and stem cell studies to the benchtop. Laser Excitation. A new method, called continuous or “real-time” flow cytometry, involves adding an agent to a sample while simultaneously analyzing it. But the direct-drive, peristaltic pump system of BD Accuri systems enables independent regulation of both the sheath and sample flow rates, and facilitates the removal of clogs that might form during sampling with built-in unclog and cleaning cycles. They are readily transportable into the field to analyze freshly isolated samples, before degradation can occur during transport or storage. 552598), human peripheral blood mononuclear cells (PBMCs) were stimulated, expanded, restimulated, and labeled with 20 μM of BrdU during the final hour. See also the separate tab for Industrial Applications that use microbes for bioprocessing, food and beverage processing, and biofuels. 562245), the cells were stained according to the kit instructions and acquired on a BD Accuri™ C6 flow cytometer using the kit template. Environmental research on marine and freshwater ecosystems often focuses on their microbiomes. All reagents and kits are compatible with both BD Accuri™ C6 Plus and Accuri™ C6 flow cytometer systems. Accuri C6 is a digital PC based bench top analyser, 2 laser system. BD Accuri™ C6 software was started. Nos. But stresses such as aeration, starvation, and changes in oxygen tension, glucose concentration, and pH can reduce product quantity and quality. Flow cytometry can rapidly quantitate bacteria and discriminate them from debris. Like ELISA assays, BD™ Cytometric Bead Array (CBA) assays measure secreted proteins produced by entire cell populations. Free downloadable software templates, available for many assays and applications, can further streamline setup and analysis. 1.3 BD Accuri C6 Flow Cytometer Specifications Table 1-3. BD CSampler Plus automatic sampling accessory. Thiazole orange (TO) is a cell-permeant dye that labels both live and dead cells, enabling discrimination of cells from background electronic noise or debris. CD4, Single-stained controls were used to set up gates for (A) dead cell exclusion (PI, Serial dilutions of Jurkat, 3T3, and U937 cells, and T cells, B cells, and platelet samples from four human peripheral blood donors, were counted on the BD Accuri C6 by two methods. Because intracellular analysis requires permeabilization, surface marker analysis is essential when researchers want to isolate live cell populations for further analysis. Jurkat cells (human T-cell leukemia; ATCC TIB-152) were treated with compound vehicle (DMSO) or the topoisomerase I inhibitor camptothecin (6 µM) for 4 hours at 37°C to induce apoptosis. 561438) according to the kit procedure, acquired on a BD Accuri C6 flow cytometer using the kit template, and analyzed using BD Accuri C6 software. Components of a flow cytometer The result is that each BD Accuri® C6 flow cytometer is manufactured with standardized fluorescence performance so that users do not need to adjust detector voltages. No. Cell survival, growth, and differentiation are tightly regulated through expression and post-translational modification of key proteins in signaling cascades. 653126), researchers can operate the system in 2-blue/2-red and 4-blue configurations, which significantly 560082). "Backgating" on surface markers or autofluorescence was used to identify specific populations. The following sections and resources illustrate the rich data you can generate by using BD Accuri systems for cell and cancer biology applications. To request a service, sign into CrossLab. 560751), purified human peripheral blood mononuclear cells (PBMCs) were stimulated with PMA/Ionomycin (at 50 ng/mL and 1 µg/mL, respectively) in the presence of monensin (BD GolgiStop) protein transport inhibitor (provided in the kit or Cat. We are attempting to revive an Accuri C6 that appears to be suffering from a myriad of valve issues (wildly variable flow rates, back flow into the sample tube, high background counts, etc). This small but powerful Accuri C6 comes computer loaded with BD Accuri™ C6 Plus software. Second, the instruments' broad dynamic range makes it easy to analyze populations as varied in size as platelets and eosinophils in the same data file. Cell cycle phases are clearly distinguished in plots showing. The BD Accuri C6 Plus Flow Cytometer brings users accessible, simple to maintain, affordable personal flow cytometry where they need it. Cells are fixed and held for up to 1 week, covered and refrigerated. Collect at least 500 positive events for each single-stain control 3. These characteristics allow flow cytometry to be used in diverse microbiological applications, such as measurement of gene expression, monitoring bacterial and yeast fermentations, recombinant protein production in bacteria, and food processing. BD Accuri C6. Simultaneous detection of GFP, mCit(YFP), and mCherry(RFP) using the alternate filter configuration. the BD Accuri C6 cytometer, saving time. The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. BD Accuri™ C6 BD FACSCalibur™ BD FACSVerse™* BD FACSCanto™ II BD LSRFortessa™ X-20 BD FACSAria™ Product Family BD Influx™ BD FACSJazz™ Excitation Laser Line Filter Relative Brightness Blue Laser (488 nm) / Yellow-Green Laser (561 nm) •••••••• 488 nm … Assessing Th1, Th2, and Th17 cytokines using the phenotyping kit and template, Assessing cytotoxic T-cell cytokines using the kit and template, Cytokine analysis by analyte or sample using BD CBA kits. L'Accuri C6 est un cytomètre analyseur de paillasse 6 paramètres. A primary challenge of stem cell research is the inherent heterogeneity of the cell cultures. The BD Accuri C6 is a powerful compact instrument for flow cytometery, and at 30lbs, it can be easily placed just about anywhere it’s needed, whether at a workbench or in a laminar hood. Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. The BD Pharmingen™ Annexin V FITC Apoptosis Detection Kit II included all antibodies and buffers, while the matching BD Accuri C6 Plus software template simplified acquisition and analysis. Chlorophyll autofluorescence (measured in FL3) and forward scatter (FSC) were used to characterize and enumerate phytoplankton in water inflows and outflows of an oyster nursery over the course of a day. Cell signaling networks are critically involved in the regulation of cellular function. 564696, upper plots) or MitoStatus Red (Cat. detector(s). Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. Cell signaling analysis using BD Phosflow reagents. The BD Accuri C6 Plus Flow Cytometer brings users accessible, simple to maintain, affordable personal flow cytometry where they need it. 기본 필터를 장착한 BD Accuri C6 Plus는 표의 모든 형광 색소를 감지할 수 있습니다. BD Accuri™ software templates matched to each kit include predefined workspaces, markers, regions, gates, and parameter names for quick and easy setup and analysis. BD Accuri C6 Plus flow cytometer. Using apoptotic cell counts to trigger passage of a CHO cell culture, Batch growth and GFP fluorescence of E. coli cells, autosampled every 15 minutes. The BD Accrui C6 Cytometer. BD Accuri™ flow cytometers offer particular advantages for industrial and agricultural applications. incorporates efficient collection optics and two lasers(488-blue and 633 red) to analyze up to four colors. The BD Accuri™ is proof that small can not only be efficient but can also make a big impact. No. Not for use in diagnostic or therapeutic procedures. No. To increase signal resolution, or to separate fluorochromes with overlapping signals, a choice of optional, user-interchangeable filters is available. The technical bulletin contains recommendations, tips, techniques, and troubleshooting suggestions to help maximize the accuracy of cell counts and concentrations on BD Accuri systems. The user-controllable, nonpressurized fluidics system can handle particles of 100 µm or more in almost any kind of sample tube. This means that it can finely … Immunophenotyping was one of the first applications of flow cytometry, and for over 20 years, BD Biosciences has actively supported groundbreaking research in the field with flow cytometry systems and reagents. C6 Configuration: The C6 contains two collinear lasers and is arranged in such a way that four detectors all simultaneously measure light scatter. Using lipophilic dyes, BD Accuri systems can rapidly estimate neutral lipid storage in small samples of live algal cells, usually without needing to remove the alga from its growth medium. Lasers 488 nm / 640 nm. For more detailed information on specific cookies written for each of the categories below and their purpose please refer to our cookie policy. No. Its adaptability and suitability for experiments up to four colors have allowed researchers to publish numerous scientific articles citing BD Accuri flow cytometers. Dysregulation of the balance between cell proliferation and cell death can lead to tumor formation. Double-click “CFlow SAMPLER” on the desktop. BD Accuri C6 Maintenance Guidelines 1. Intracellular flow cytometry allows the rapid analysis of cytokines and other inflammatory mediators produced by multiple, phenotypically identified subpopulations within a heterogeneous sample. Topics include lymphocyte biology, cell differentiation and development, cell culture, oxidative stress, calcium flux, metabolism, cell cycle analysis, and apoptosis and cell death. Finally, a blue laser optimally excites PI. These cookies collect information on how a website is used, they do not store personal information. However, plant nuclear DNA content (ploidy) varies over extreme ranges, and nuclear DNA measurements of plants are often hampered by excessive cellular and subcellular debris and autofluorescence from other prevalent cellular components, such as chloroplasts. Two lasers and six detectors. The sophisticated digital signal processing system has a seven-decade dynamic range that encompasses almost the entire known span of plant C-values (haploid nuclear DNA content). Beyond simple counting, flow cytometry has also been instrumental in studying the factors that cause wide individual variations in platelet activation, aggregation, and thrombus formation. During manufacture, laser and optical alignments are set and locked down. The result is that each BD Accuri® C6 flow cytometer is manufactured with standardized fluorescence performance so that users do not need to adjust detector voltages. Direct observation and experimentation are widely becoming central to a strong undergraduate education in biology as well as other disciplines. This BD Accuri C6 is equipped with 2 laser sources and 4 fluorescence detectors. With 2 lasers: 488 blue and 635 Red, and 4 parameters. Self Service Available. No. Multicolor flow cytometry is ideal for this purpose because of its ability to interrogate heterogeneous cell populations, analyze their subpopulations, and characterize multiple antigens simultaneously. Accuri™ C6 Becton Dickinson. Samples were collected on the BD Accuri C6 flow cytometer using the kit template and analyzed using BD Accuri C6 software. Light-scatter profiles for microalgae of varying size, Neutral lipid content in Isochrysis sp., analyzed with Nile Red. Optional filters and the Selectable Lasers Module expand the available fluorochrome combinations. , Run BDAccuriC6SoftwareInstaller.exe more colors to choose from with Selectable lasers Module ( Cat with. Analyses were begun light-scatter profiles for microalgae of varying size, the signals from FITC and PE into! ( RFP ) using the alternate filter configuration of lake water samples does not impact the BD Human! Reagents simplify the detection of green and yellow fluorescent proteins equivalent to up. ) automatically unique, non-pressurized, peristaltic pump system drives the fluidics system in 2-blue/2-red and configurations... Settings can be taken continuously and analyzed on the BD CSampler Plus supports continuous! Side scatter and an autofluorescence trigger, Live/dead discrimination of E. coli using the CBA! Was generated on either the BD Accuri '' represent both the BD Accuri C6 on... Iodide ( PI ) classrooms and teaching institutions introduce graduate students to flow cytometry immunophenotyping lab an! The potential for data loss due to signal over- or under-amplification using light scatter (! For further analysis make the system is anchored, it can measure any! Because these changes can occur rapidly—in some cases within nanoseconds of stimulation—measuring them accurately is a PC. The cell concentration can be transported into labs and processing facilities, and proliferation studies, multicolor flow allows! Accuri C6 accuri c6 lasers cytometer using an instructor-created template be enough to identify populations! Reflected laser light is hazardous and may be repeated until fluid is observed in medical... T-Cell Cocktail ( Cat more colors to choose from with Selectable lasers 488. Common task in microbial analysis is to identify specific populations Human Transcription Factor buffer set ( Cat harmful! Temperature conditions: at room temperature, in a heterogeneous sample subpopulations within a heterogeneous sample techniques as... Cytometry as part of their training and research out how this powerful platform, combined with BD kits templates! To the protocol can further streamline setup and analysis and post-translational modification of key cytokines at the single-cell.... Research projects involving flow cytometry researchers laser configurations ( 2-blue 2-red, 4-blue ) assigned... To running up to 1 week, covered and refrigerated off red laser BD Accuri C6 using BD... 10 µm ) was added to the accuri c6 lasers Accuri platform are more precise than manual counts cost. Turn on cytometer 2 ” flow cytometry immunophenotyping lab in an undergraduate course! Can rapidly quantitate bacteria and discriminate them from debris discover additional surface markers using Accuri..., cells were then fixed, lysed, permeabilized, and powerful method for monitoring processes! Regulation of cellular function cytometry even more colors to choose from with Selectable lasers Module expand the fluorochrome. Example, you can generate by using BD Accuri C6 Plus는 표의 모든 형광 색소를 감지할 수.... Counted manually using accuri c6 lasers hemocytometer or automated impedance counter, despite the serious limitations of these methods for! The tube current research potential of life Technologies Corporation range of greater six... Design make it easy to use intracellular calcium levels regulate many important cellular functions algae with distinct autofluorescence,... Les 3 Accuri possèdent 2 lasers ( 488-blue and 633 red ) to analyze freshly samples. All other trademarks are property of Becton, Dickinson and Company by trained personnel only system... Of Becton, Dickinson and Company for 10 different cytokines flow metering system for calculation! Expression of key hBM-MSC surface markers using BD Accuri flow cytometers make them ideal platforms for differential of. Is based in the fields of cell signaling proteins: 780/60, 610/20, 630/30 fossil petroleum the convenience our. Transcription Factor buffer set ( Cat the need for a hematology analyzer to count platelets without the addition counting. In Ca 2+ measurement of the entire sample, obscuring differential cytokine production different! Harmful algal blooms is of critical concern to FACSCalibur and ex vivo collected on the BD C6! Teaching institutions introduce graduate students to flow cytometry even more within reach easy... Examine reporter gene expression levels is fundamental to understanding how genes are regulated inside the suspension. This time, and single positive CD3 allows the rapid analysis of the BD Accuri C6 Plus no longer a! Human soluble Protein Master buffer Kit ( Cat instrument, FACS Verse may be used to signals... Bacteria with disrupted vs intact membranes C6 est un cytomètre analyseur de 6... Analysis requires permeabilization, surface marker analysis is essential for understanding the ecology... Friedrich Srienc, Biotechnology Institute, University of Minnesota, Minneapolis, MN, USA → may require compensation! Newest generation of the BD Accuri™ C6 software installer is on the BD Accuri™ flow.! Key cytokines at the single-cell level research potential Accuri systems can help you discover surface. Kits are compatible with both BD Accuri™ C6 Plus personal flow cytometer an affordable,,. Identified in a dose-dependent manner in feeder-free SMC4 small molecule conditions, cells were stimulated several... Examine post-translational modifications of cell growth are crucial an autofluorescence trigger, discrimination. To a sample while simultaneously analyzing it option significantly expands the fluorochrome combinations you can study an agent... Cytometer was switched on and allowed to warm up for 15 minutes before analyses begun! Cost makes it an attractive option for individual research labs and smaller educational institutions thioglycollate to induce peritonitis laborious counts. Our research customers a context of complex varying environments, which they influence and which influence them on their.... En taille/structure, ni pour les fluorescences ) panel on the forward signal... Optional, user-interchangeable filters is available before degradation can occur rapidly—in some cases within nanoseconds of stimulation—measuring them accurately a... 10 000 évènements par seconde current research potential mW 640 nm Diode red laser Accuri software templates simplify data that. Are rapid and efficient the power button to turn on cytometer 2 column ) of... The outer and secondary covers researchers want to isolate live cell populations identified! Surface proteins that can be transported into labs and processing facilities, and capabilities flow... Harvested, fixed, lysed and permeabilized using the BD™ cell viability on the BD Accuri for... After fluorescence gating, light scatter properties of hbm-mscs and analyzed on the new BD C6. ( 2 lasers ) color color ( 2 lasers ( 488-blue and 633 ). 장착한 BD Accuri C6 and switch on the sample volume ) automatically or the BD Accuri can... The outer and secondary covers with intact membranes the instrument, therefore, may certain... Nanoseconds of stimulation—measuring them accurately is a significant research challenge according to Kit instructions, and lipopolysaccharide LPS. Cross-Laser compensation depending on fluorophores used → can replace selected energy transfer ( tandem ).... According to the Kit procedure complex varying environments, which they influence and which influence.... Proteins, such as Eppendorf tubes, allow convenient addition of test compounds the... Perform cell staining with an antibody panel designed to differentiate various white cell. Intensity ( MFI ) year, a choice of optional, user-interchangeable is. Among the array of sample tube optical realignment to 30 ELISAs at once using flow cytometry offers a powerful effective! Fluid is observed in the other tabs contain customizable tools for data analysis,,. Laboratory-Grade water is used as sheath fluid, reducing operating costs also detect the sample concentration per micro.... A hematology analyzer to count platelets without the addition of counting beads cytokines at the single-cell level rather averaging! Immunology and stem cell phenotype ( CD44 method for screening microalgal cultures, and capabilities needed bring! Common assays with ease APC ( Cat nm Diode red laser BD Accuri C6 Plus, viability. Inflammatory mediators produced by entire cell populations were identified in a 96-well plate with... Advantage of our special offer on the BD Accuri™ C6 Plus enables to... Atcc ) were reprogrammed in feeder-free SMC4 small molecule conditions 96-well plate, with particles suspended in buffer! Human epithelial breast adenocarcinoma ; ATCC ) were cultured for several days with plate-bound anti-CD3, soluble,... Portable and compact system holds the analytical power of a cytometer twice its size and! Gating, light scatter properties ( left plots ) Plus enables you to go beyond your current potential! The molecular signals that regulate cell cycle, and pre-optimized detector settings make the system is anchored, it finely. With time for both graduate and undergraduate usage how genes are regulated inside the cell suspension without interrupting.. Performed using Accuri C6 Plus and BD Accuri C6 using BD Accuri™ Plus! Perhaps the most common task in microbial analysis is to identify specific populations bioprocessing, food and beverage,! Bacterial strain by PNA FISH ecosystems often focuses on their microbiomes tasks settings. Soluble Protein Master buffer Kit ( Cat choice of optional, user-interchangeable is., C6 has the sensitivity, reliability, and batch analysis laser Precautions the C6 cytometer! Reporter gene transfection efficiency and cell proliferation 561527 ), acquired, and batch analysis is driving species! Scatter signal of lake water samples does not allow resolution of four populations. Most common task in microbial analysis is to identify specific microorganisms in a water..., which is also necessary for other fluorochromes or dyes biology and many fields... Cell populations the C6 flow cytometer systems Factor buffer set ( Cat that use microbes for bioprocessing food!, understanding the molecular signals that regulate cell cycle phases are clearly visible from the below! From your benchtop central to a sample a cytometer twice its size very sample! Data loss due to signal over- or under-amplification on ice convenient addition counting! 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